| Refrigerated
doughs are susceptible to spoilage of species of the
bacteria Lactobacillus and Leuconostoc that
can grow at low temperatures (especially at 10oC and
above) producing CO2 and causing the containers
to blow. Wild yeast may also be present in low numbers.
Breads usually have
low moisture which prevent the growth of most bacteria but
not of molds. Spores of the bacterium Bacillus subtilis can
survive baking and can grow and produce "ropiness" (soft,
stringy, brown mass with fruity odor. Mold spores are
killed by baking but the bread is contaminated by spores
from the air or equipment. A common mold is Rhizopus
stolonifer.
Most pastries, due
to the low water content, will only allow molds to grow. However,
they can be spoiled by microorganisms found in icings, nuts,
fillings, toppings, and creams added after baking. Some
fillings with pH>4.0 and water activity (aw )
greater than 0.85 must be considered potentially hazardous
and treated as perishable food ,i.e., they must be refrigerated.
Dry pastas do not
support growth of microorganisms but fresh pastas can be
spoiled by bacteria, yeasts, and molds. Several pasta
products have been recalled due to the presence of Salmonella. Fresh
egg noodles may have significant numbers of staphylococci
and enteric streptococci. Enterotoxin production by S.
aureus is a potential health hazard. This toxin
will not be inactivated during cooking. Good sanitary
practices are very important in the production of fresh pastas.
Cereal grains normally
have only 10-12% moisture; however, during harvesting, processing,
and storage this moisture can be higher and some molds can
grow (Aspergillus, Penicillium, Cladosporium, Fusarium,
Alternaria, and Rhizopus). Some molds such
as Aspergillus flavus and A. parasiticus can
grow during storage. These molds produce mycotoxins such
as aflatoxins, and present a potential health hazards to consumers. External
contaminants such as coliforms, E. coli, and enterococci
may be contributed by birds, insects, and rodents. Storage
conditions (moisture, temperature and time) are critical factors
in controlling these microorganisms.
Flour may contain large
quantities of microorganisms such as rope-forming spores as
well as psychrotrophs, flat-sour organisms, thermophilic sporeformers,
and molds such as Penicillium, Aspergillus, Cladosporium,
Mucor, and Rhizopus. Soy flour is manufactured by
a different procedure and has a higher moisture content than
wheat flour. Salmonella spp. have been detected
often in soy flour.
Ready-to-eat cereals, when
properly processed and stored, do not support the growth of
bacteria, yeasts or molds.
Good manufacturing practices and
good sanitation practices can control and even reduce the number
of pathogenic and spoilage microorganisms in cereal grains
and their products during processing and storage. Wet
cleaning should be avoided whenever possible since it usually
causes more problems that it solves due to an increase in moisture
level. Rigid application of a Hazard Analysis Critical
Control Point (HACCP) system is highly recommended.
The aerobic plate count, coliform
count, and yeast and mold count are important indices of good
sanitation, handling, processing, and storage practices. Other
recommended tests for products such as pasta are: E.
coli, staphylococci count and Salmonella test.
Recommended tests:
Aerobic plate count.
Yeast and Molds count.
Total coliforms and E. coli.
Staphylococci.
Salmonella.
Additional tests:
Rope spores (cereal grains and
flours).
Mycotoxins.
Staphylococcal enterotoxin.
Clostridium perfringens )in soy products).
Bacillus cereus (in rice)
Adapted from:
Deibel, K. E. and Swanson, K.
M. 2001. Cereals and Cereal Products, p. 549. In
F. P. Downes and K. Ito (eds.), Compendium of Methods for the
Microbiological Examination of Foods. American Public
Health Association, Washington, DC.
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